|ITEM METADATA RECORD
|Title: ||Impaired Islet Function in Commonly Used Transgenic Mouse Lines due to Human Growth Hormone Minigene Expression|
|Authors: ||Brouwers, Bas|
de Faudeur, Geoffroy
Osipovich, Anna B.
Cauwelier, Elisa J.G.
Van Lommel, Leentje
Van Schoors, Jolien
Stancill, Jennifer S.
in't Veld, Peter
Magnuson, Mark A.
Schuit, Frans ×
Schraenen, Anica #
|Issue Date: ||2-Dec-2014 |
|Publisher: ||Cell Press|
|Series Title: ||Cell Metabolism vol:20 issue:6 pages:979-990|
|Article number: ||S1550-4131(14)00501-4|
|Abstract: ||The human growth hormone (hGH) minigene is frequently used in the derivation of transgenic mouse lines to enhance transgene expression. Although this minigene is present in the transgenes as a secondcistron, and thus not thought to be expressed, we found that three commonly used lines, Pdx1-Cre(Late), RIP-Cre, and MIP-GFP, each expressed significant amounts of hGH in pancreatic islets. Locally secreted hGH binds to prolactin receptors on β cells, activates STAT5 signaling, and induces pregnancy-like changes in gene expression, thereby augmenting pancreatic β cell mass and insulin content. In addition, islets of Pdx1-Cre(Late) mice have lower GLUT2 expression and reduced glucose-induced insulin release and are protected against the β cell toxin streptozotocin. These findings may be important when interpreting results obtained when these and other hGH minigene-containing transgenic mice are used.|
|Publication status: ||published|
|KU Leuven publication type: ||IT|
|Appears in Collections:||Gene Expression Unit|
Department of Human Genetics - miscellaneous
Laboratory for Biochemical Neuroendocrinology
Translational Research in GastroIntestinal Disorders
× corresponding author|
# (joint) last author|
|Files in This Item:
|Impaired Islet Function in Commonly Used Transgenic Mouse Lines due to Human Growth Hormone Minigene Expression.pdf||Article||