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Title: Impaired Islet Function in Commonly Used Transgenic Mouse Lines due to Human Growth Hormone Minigene Expression
Authors: Brouwers, Bas
de Faudeur, Geoffroy
Osipovich, Anna B.
Goyvaerts, Lotte
Lemaire, Katleen
Boesmans, Leen
Cauwelier, Elisa J.G.
Granvik, Mikaela
Pruniau, Vincent
Van Lommel, Leentje
Van Schoors, Jolien
Stancill, Jennifer S.
Smolders, Ilse
Goffin, Vincent
Binart, Nadine
in't Veld, Peter
Declercq, Jeroen
Magnuson, Mark A.
Creemers, John
Schuit, Frans ×
Schraenen, Anica #
Issue Date: 2-Dec-2014
Publisher: Cell Press
Series Title: Cell Metabolism vol:20 issue:6 pages:979-990
Article number: S1550-4131(14)00501-4
Abstract: The human growth hormone (hGH) minigene is frequently used in the derivation of transgenic mouse lines to enhance transgene expression. Although this minigene is present in the transgenes as a secondcistron, and thus not thought to be expressed, we found that three commonly used lines, Pdx1-Cre(Late), RIP-Cre, and MIP-GFP, each expressed significant amounts of hGH in pancreatic islets. Locally secreted hGH binds to prolactin receptors on β cells, activates STAT5 signaling, and induces pregnancy-like changes in gene expression, thereby augmenting pancreatic β cell mass and insulin content. In addition, islets of Pdx1-Cre(Late) mice have lower GLUT2 expression and reduced glucose-induced insulin release and are protected against the β cell toxin streptozotocin. These findings may be important when interpreting results obtained when these and other hGH minigene-containing transgenic mice are used.
URI: 
ISSN: 1550-4131
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Gene Expression Unit
Department of Human Genetics - miscellaneous
Laboratory for Biochemical Neuroendocrinology
Translational Research in GastroIntestinal Disorders
× corresponding author
# (joint) last author

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