Title: Expression of human fibroblast interferon gene in Escherichia coli
Authors: Derynck, R ×
Remaut, E
Saman, E
Stanssens, P
De Clercq, Erik
Content, J
Fiers, W #
Issue Date: Sep-1980
Publisher: Nature Publishing Group
Series Title: Nature vol:287 issue:5779 pages:193-7
Abstract: The human fibroblast interferon gene was inserted in a thermoinducible expression plasmid under control of the phage lambda PL promoter. The primary translation products predicted on the basis of the plasmid constructions were hybrid proteins starting with beta-lactamase or phage MS2 polymerase information followed by the total preinterferon. On induction, antiviral activity, whose physico-chemical, immunological and biological characteristics closely corresponded to those of authentic human fibroblast interferon, was synthesized. Processing to a size compatible with mature but unglycosylated authentic product was observed.
ISSN: 0028-0836
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Virology and Chemotherapy (Rega Institute)
× corresponding author
# (joint) last author

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