Title: Assay to probe proteolytic processing of Notch by γ-secretase
Authors: Serneels, Lutgarde ×
Tesseur, Ina
De Strooper, Bart #
Issue Date: 2014
Publisher: Humana Press, Inc.
Series Title: Methods in Molecular Biology vol:1187 pages:223-9
Article number: 10.1007/978-1-4939-1139-4_17
Abstract: With the increasing appreciation of the role of Notch in development and disease, measuring its cleavage and signaling activity in cellular systems has become important. Here we describe a cell-based method to analyze the cleavage of Notch at the S3 site by γ-secretase. HEK cells are transfected with an N-terminal truncated and myc-labeled mNotchΔE construct which can be easily and quantitatively detected by western blotting.
ISSN: 1064-3745
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Department of Human Genetics - miscellaneous
Laboratory for the Research of Neurodegenerative Diseases
× corresponding author
# (joint) last author

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