Title: Screening for Bacillus subtilis mutants deficient in pressure induced spore germination: identification of ykv U as a novel germination gene
Authors: Aertsen, Abram ×
Van Opstal, Isabelle
Vanmuysen, SC
Wuytack, EY
Michiels, Christiaan #
Issue Date: 2005
Publisher: Elsevier on behalf of the Federation of European Microbiological Societies
Series Title: FEMS Microbiology Letters vol:243 issue:2 pages:385-391
Abstract: Exposure to high pressure induces germination in spores of Bacillus subtilis. To investigate the mechanisms of this process and to compare the pressure and nutrient induced germination pathways, a random transposon knock-out library of B. subtilis was constructed and screened for clones with a compromised pressure induced germination at 100 MPa. Two mutants were isolated and their transposon insertion was mapped to gerAC and ykv U respectively. While GerAC is required for production of the L-alanine receptor which has been implicated in pressure-induced germination before, YkvU is shown here to be a novel germination determinant in B. subtilis, affecting germination by high (100 MPa) and very high (600 MPa) pressure, by nutrients and by dodecylamine, but not by Ca2+-dipicolinic acid. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
ISSN: 0378-1097
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre for Food and Microbial Technology
× corresponding author
# (joint) last author

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