Title: Endoxylanase substrate selectivity determines degradation of wheat water-extractable and water-unextractable arabinoxylan
Authors: Moers, Karolien ×
Celus, Inge
Brijs, Kristof
Courtin, Christophe
Delcour, Jan #
Issue Date: 2005
Publisher: Elsevier sci ltd
Series Title: Carbohydrate research vol:340 issue:7 pages:1319-1327
Abstract: The relative activity of all endoxylanase towards water-unextractable (WU-AX) and water-extractable arabinoxylan (WE-AX) substrates, referred to as endoxylanase substrate selectivity, impacts the enzyme functionality in cereal-based biotechnological processes such as bread-making and gluten starch separation. A set of six endoxylanases representing a range of substrate selectivities as determined by a screening method using chromophoric Substrates [Anal. Biochem. 2003, 319, 73-77] was used to examine the impact of such selectivity on changes in structural characteristics of wheat WU-AX and WE-AX upon enzymic hydrolysis. While WE-AX degradation by the selected endoxylanases was very comparable with respect to apparent molecular mass (MM) profiles and arabinose to xylose ratio of the hydrolysates formed, WU-AX solubilisation and Subsequent degradation of solubilised fragments gave rise to widely varying MM profiles, depending on the substrate selectivity of the enzymes. Enzymes with high selectivity towards WU-AX de facto generated higher MM fragments from WU-AX than enzymes with low selectivity. The arabinose to xylose ratios of solubilised fragments were independent of the degree of solubilisation. (c) 2005 Elsevier Ltd. All rights reserved.
ISSN: 0008-6215
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre for Food and Microbial Technology
× corresponding author
# (joint) last author

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