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Title: Digital Droplet PCR on Chip for Quantitative Assesement of MicroRNAs
Authors: Cai, Qing
Wiederkehr, Rodrigo Sergio
Jones, Ben
Majeed, Bivragh
Colle, Frederick
Zhang, Lei
Stakenborg, Tim
Fiorini, Paolo
Lagae, Liesbet
Tsukuda, Masahiko
Matsuno, T
Yamashita, Ishiro
Issue Date: 26-Oct-2014
Publisher: The Chemical and Biological Microsystems Society
Host Document: Proceedings of the 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences pages:1015-1017
Conference: International Conference on Miniaturized Systems for Chemistry and Life Sciences edition:18th location:San Antonio, TX USA date:26-30 October 2014
Abstract: In this paper, the layout, fabrication and preliminary testing of a microfluidic chip for the quantitative detection of microRNA (miRNA) molecules is described. The chip integrates the following steps for miRNA quantification: miRNA release, reverse transcription and digital droplet PCR. The experiments for quantifying let-7a miRNA copy numbers were performed using MCF-7 cells. Results show that the digital on-chip and analog off-chip quantifications are in good agreement; the determined concentration on the same sample was 780 templates/L and 900 template/l, respectively.
ISBN: 978-0-9798064-7-6
Publication status: published
KU Leuven publication type: IC
Appears in Collections:Solid State Physics and Magnetism Section

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