Proceedings of the 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences pages:1015-1017
International Conference on Miniaturized Systems for Chemistry and Life Sciences edition:18th location:San Antonio, TX USA date:26-30 October 2014
In this paper, the layout, fabrication and preliminary testing of a microfluidic chip for the quantitative detection of microRNA (miRNA) molecules is described. The chip integrates the following steps for miRNA quantification: miRNA release, reverse transcription and digital droplet PCR. The experiments for quantifying let-7a miRNA copy numbers were performed using MCF-7 cells. Results show that the digital on-chip and analog off-chip quantifications are in good agreement; the determined concentration on the same sample was 780 templates/L and 900 template/l, respectively.