Title: A Compact Device for Digital Droplet PCR
Authors: Wiederkehr, Rodrigo Sergio
Jones, Ben
Majeed, Bivragh
Colle, Frederick
Chen, Wei-Chen
Stakenborg, Tim
Fiorini, Paolo
Lagae, Liesbet
Schimdt, Kristiane
Stuyver, Lieven
Issue Date: 26-Oct-2014
Publisher: The Chemistry and Biological Microsystems Society
Host Document: Proceedings of the 18th International Conference on Miniaturized Systems for Chemistry and Life Science pages:1000-1002
Conference: International Conference on Miniaturized Systems for Chemistry and Life Science (Microtas 2014) edition:18th location:San Antonio, TX USA date:26-31 October 2014
Abstract: In this work, the fabrication and testing of a miniaturized device to perform all steps necessary for
digital droplet polymerase chain reaction (ddPCR) within a single chip is presented. The microfluidic
chip, made in silicon, contains a T-junction for droplet generation along with a microreactor for
temperature cycling. The temperature is controlled by means of an external, miniaturized thermoelectric
element. The number of fluorescent droplets after PCR amplification is determined using a fluorescent
microscope; furthermore, individual droplet fluorescence is recorded at each cycle, thus enabling real
time PCR analysis. The microreactor accommodates about 2500-3000 droplets of uniform volume
varying in the 55-65 pL range, allowing the direct and accurate detection of human genomic DNA in
concentrations from 0.06 to 40 ng/μL.
Publication status: published
KU Leuven publication type: IC
Appears in Collections:Solid State Physics and Magnetism Section

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