Effect of milrinone analogues on intracellular calcium increase in single living H9C2 cardiac cells
Pietrangelo, Tiziana Giampietro, Letizia De Filippis, Barbara La Rovere, Rita Fulle, Stefania Amoroso, Rosa # ×
European Journal of Medicinal Chemistry vol:45 issue:11 pages:4928-33
The synthesis of milrinone analogues where the 4-pyridyl moiety was replaced by an ester or amide group is reported. Only amide derivatives are able to support intracellular calcium influx following chemical depolarization with 60 mM KCl in a percentage varying from 20 to 45% of differentiated H9C2 cardiomyocytes. Those cells were differentiated after chronic exposure to 10 nM retinoic acid which induces the expression of voltage-gated calcium channels. Analogues of milrinone containing an ester function did not show significant activity.