Title: Measurement of two-photon excitation spectrum used to photoconvert a fluorescent protein (Kaede) by nonlinear Fourier-transform spectroscopy
Authors: Isobe, Keisuke
Hashimoto, Hiroshi
Suda, Akira
Kannari, Fumihiko
Kawano, Hiroyuki
Mizuno, Hideaki
Miyawaki, Atsushi
Midorikawa, Katsumi # ×
Issue Date: 2010
Series Title: Biomedical Optics Express vol:1 issue:2 pages:687-693
Abstract: We demonstrate the measurement of two-photon excitation (TPE) spectra, used not only for fluorescence but also for photoconversion in green-to-red photoconvertible Kaede, using nonlinear Fourier-transform spectroscopy. It was found that in unphotoconverted Kaede, the TPE spectrum for photoconversion is much different to that for green-fluorescence. This is similar to the difference between the one-photon excitation of photoconversion in the neutral form and that of green-fluorescence in the ionized form.
ISSN: 2156-7085
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry, Molecular and Structural Biology Section
× corresponding author
# (joint) last author

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