Title: High-resolution fluorescence microscopy based on a cyclic sequential multiphoton process
Authors: Isobe, Keisuke
Suda, Akira
Hashimoto, Hiroshi
Kannari, Fumihiko
Kawano, Hiroyuki
Mizuno, Hideaki
Miyawaki, Atsushi
Midorikawa, Katsumi # ×
Issue Date: 2010
Series Title: Biomedical Optics Express vol:1 issue:3 pages:791-797
Abstract: We demonstrate high-resolution fluorescence microscopy based on a cyclic sequential multiphoton (CSM) process, which gives rise to fluorescence emission following a sequence of cyclic transitions between the bright and dark states of a fluorophore induced by pump and reverse light. By temporally modulating the reverse intensity, we can extract the fluorescence signal generated through the CSM process. We show that the demodulated fluorescence signal is nonlinearly proportional to the excitation intensities and it gives a higher spatial resolution than that of a confocal microscope.
ISSN: 2156-7085
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry, Molecular and Structural Biology Section
× corresponding author
# (joint) last author

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