Title: Probing Ambiguous Base-Pairs by Genetic Transformation with XNA Templates
Authors: Pezo, Valérie
Schepers, Guy
Lambertucci, Catia
Marlière, Philippe
Herdewijn, Piet # ×
Issue Date: Oct-2014
Publisher: Wiley-VCH
Series Title: Chembiochem vol:15 issue:15 pages:2255-8
Abstract: The templating potential of anhydrohexitol oligonucleotides bearing ambiguous bases was studied in vivo, by using a selection screen for mosaic heteroduplex plasmids in Escherichia coli. 1,5-Anhydro-2,3-dideoxy-2-(5-nitroindazol-1-yl)-D-arabino-hexitol showed the greatest ambiguity among the three nucleosides tested. At most two successive ambiguous bases could be tolerated on hexitol templates read in bacterial cells. Hexitol nucleosides bearing simplified heterocycles thus stand as promising monomers for generating random DNA sequences in vivo from defined synthetic oligonucleotides.
ISSN: 1439-4227
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Medicinal Chemistry (Rega Institute)
× corresponding author
# (joint) last author

Files in This Item:
File Description Status SizeFormat
031114 - Lirias post-print.docxOA article Published 368KbUnknownView/Open Request a copy
031114 - Lirias publishers pdf.pdfOA Article Published 372KbAdobe PDFView/Open Request a copy

These files are only available to some KU Leuven Association staff members


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science