Probing Ambiguous Base-Pairs by Genetic Transformation with XNA Templates
Pezo, Valérie Schepers, Guy Lambertucci, Catia Marlière, Philippe Herdewijn, Piet # ×
Chembiochem vol:15 issue:15 pages:2255-8
The templating potential of anhydrohexitol oligonucleotides bearing ambiguous bases was studied in vivo, by using a selection screen for mosaic heteroduplex plasmids in Escherichia coli. 1,5-Anhydro-2,3-dideoxy-2-(5-nitroindazol-1-yl)-D-arabino-hexitol showed the greatest ambiguity among the three nucleosides tested. At most two successive ambiguous bases could be tolerated on hexitol templates read in bacterial cells. Hexitol nucleosides bearing simplified heterocycles thus stand as promising monomers for generating random DNA sequences in vivo from defined synthetic oligonucleotides.