Title: Natural and modified promoters for tailored metabolic engineering of the yeast Saccharomyces cerevisiae
Authors: Hubmann, Georg
Thevelein, Johan
Nevoigt, Elke
Issue Date: 2014
Publisher: Humana Press, Springer, USA
Series Title: Methods in Molecular Biology,
Host Document: 'Yeast Metabolic Engineering: Methods and Protocols' edition:1152 pages:17-42
Article number: 2
Abstract: The ease of highly sophisticated genetic manipulations in the yeast Saccharomyces cerevisiae has initiated numerous initiatives towards development of metabolically engineered strains for novel applications beyond its traditional use in brewing, baking, and wine making. In fact, baker's yeast has become a key cell factory for the production of various bulk and fine chemicals. Successful metabolic engineering requires fine-tuned adjustments of metabolic fluxes and coordination of multiple pathways within the cell. This has mostly been achieved by controlling gene expression at the transcriptional level, i.e., by using promoters with appropriate strengths and regulatory properties. Here we present an overview of natural and modified promoters, which have been used in metabolic pathway engineering of S. cerevisiae. Recent developments in creating promoters with tailor-made properties are also discussed.
ISBN: 978-1-4939-0563-8
Publication status: published
KU Leuven publication type: IHb
Appears in Collections:Molecular Microbiology and Biotechnology Section - miscellaneous

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