Title: Real-Time Monitoring of Aptamer Functionalization and Detection of AraH1 by Electrochemical Impedance Spectroscopy and Dissipation-Mode Quartz Crystal Microbalance
Authors: Peeters, Marloes ×
Jiménez Monroy, Katia
Libert, Cédric
Eurlings, Yannick
Cuypers, Wim
Wackers, Gideon
Duchatau, Stijn
Robaeys, Pieter
Nesládek, Milos
van Grisven, Bart
PĂ©rez Ruiz, Elena
Lammertyn, Jeroen
Losada Pérez, Patricia
Wagner, Patrick Hermann #
Issue Date: 21-Jul-2014
Publisher: Omics Publishing Group
Series Title: Journal of Biosensors & Bioelectronics vol:5 issue:3
Article number: 1000155
Abstract: Peanut allergy, the most common cause of fatal-food-related anaphylaxis, is a lifelong disorder and the only existing therapy
is avoidance of allergen-containing food. Detection of Ara h 1, the most important peanut allergen, is commonly performed by immunoassay
techniques relying on the use of expensive and relatively unstable antibodies. Aptamers can overcome these drawbacks
and offer a great potential for the development of reliable biosensors. Therefore, we will present a novel aptamer-based sensor for
the label-free detection of Ara h 1. Amino (NH2
)-terminated Ara h 1 aptamers were covalently attached to carboxylated gold surfaces
employing carbodiimide chemistry. This functionalization procedure was followed in real time by electrochemical impedance spectroscopy
and quartz crystal microbalance with dissipation monitoring. Subsequently, the functionalized surfaces were exposed to Ara
h 1 solutions in TGK buffer. By combining the two techniques, we can measure in a wide concentration regime varying from the low
nanomolar range (1-15 nM) via electrochemical impedance spectroscopy to the higher concentrations (25-250 nM) by microgravimetric
detection. In summary, a fast, low-cost and sensitive sensor platform for Ara h 1 detection has been developed, which can be
operated as a ‘stand-alone device’, making it well suited for applications such as the screening of trace allergens.
ISSN: 2155-6210
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Mechatronics, Biostatistics and Sensors (MeBioS)
Soft Matter and Biophysics
× corresponding author
# (joint) last author

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