Title: Determination of selected protoporphyrins in Parma ham with use of 5,10,15,20-tetra(4-hydroxyphenyl)porphyrin as a surrogate standard in the recovery study
Authors: De Maere, Hannelore ×
De Mey, Eveline
Baca, Martyna
Sajewicz, Mieczysław
Paelinck, Hubert
Fraeye, Ilse
Kowalska, Teresa #
Issue Date: 2014
Publisher: Slovenian Chemical Society
Series Title: Acta Chimica Slovenica vol:61 issue:4 pages:771-777
Abstract: A high-performance liquid chromatographic analysis for the determination of hemin, protoporphyrin IX (PPIX), and zinc(II)protoporphyrin IX (Zn(II)PPIX) in Parma ham was developed. The detection was done by means of a universal DAD-detector, whereby quantification of the three naturally occurring protoporphyrins was carried out at λ = 414 nm, i.e., very close to the respective maxima of their Soret bands. The extraction thereof from the meat matrix was done by a mixture of acetone and chloroacetic acid (100 mL + 0.2 g). Usage of 5,10,15,20-tetra(4-hydroxyphenyl)porphyrin (THPP) as a surrogate standard and its detection fixed at λ = 444 nm, allowed to obtain accurate (ca. 96%) recovery results. Established concentrations of hemin, Zn(II)PPIX, and PPIX in the Parma ham samples were 15.97, 19.96 and 1.52 µg g-1, respectively.
ISSN: 1318-0207
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Bioengineering Technology TC, Technology Campuses Ghent and Aalst
Technologiecluster Bioengineering Technologie
× corresponding author
# (joint) last author

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