Author:

Keywords:

Science & Technology, Life Sciences & Biomedicine, Biochemistry & Molecular Biology, Biology, Cell Biology, Life Sciences & Biomedicine - Other Topics, INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR, CALCIUM-RELEASE, TRISPHOSPHATE RECEPTOR, ARACHIDONIC-ACID, ACINAR-CELLS, CHANNELS, ACTIVATION, MECHANISM, INS(1,4,5)P-3, MODULATOR, Animals, Boron Compounds, Calcium, Calcium Channel Blockers, Calcium Channels, Calcium Signaling, Inositol 1,4,5-Trisphosphate, Inositol 1,4,5-Trisphosphate Receptors, Ion Transport, Macrocyclic Compounds, Mice, Oxazoles, Receptors, Cytoplasmic and Nuclear, 0601 Biochemistry and Cell Biology, 0606 Physiology, 1116 Medical Physiology, 3101 Biochemistry and cell biology, 3208 Medical physiology

Abstract:

Since its introduction to Ca2+ signaling in 1997, 2-aminoethoxydiphenyl borate (2-APB) has been used in many studies to probe for the involvement of inositol 1,4,5-trisphosphate receptors in the generation of Ca2+ signals. Due to reports of some nonspecific actions of 2-APB, and the fact that its principal antagonistic effect is on Ca2+ entry rather than Ca2+ release, this compound may not have the utility first suggested. However, 2-APB has thrown up some interesting results, particularly with respect to store-operated Ca2+ entry in nonexcitable cells. These data indicate that although it must be used with caution, 2-APB can be useful in probing certain aspects of Ca2+ signaling.