Title: Diversity of dechlorination pathways and organohalide respiring bacteria in chlorobenzene dechlorinating enrichment cultures originating from river sludge
Authors: Vandermeeren, Pieter ×
Herrmann, Steffi
Cichocka, Danuta
Busschaert, Pieter
Lievens, Bart
Richnow, Hans-Hermann
Springael, Dirk #
Issue Date: Sep-2014
Publisher: Kluwer Academic Publishers
Series Title: Biodegradation vol:25 issue:5 pages:757-776
Abstract: Anaerobic reductive dechlorination of hexachlorobenzene (HCB) and three isomers of tetrachlorobenzene (TeCB) (1,2,3,4-, 1,2,3,5- and 1,2,4,5-TeCB) was investigated in microcosms containing chloroaromatic contaminated river sediment. All chlorobenzenes were dechlorinated to dichlorobenzene (DCB) or monochlorobenzene. From the sediment, a methanogenic sediment-free culture was obtained which dechlorinated HCB, pentachlorobenzene, three TeCB isomers, three trichlorobenzene (TCB) isomers (1,2,3-, 1,2,4- and 1,3,5-TCB) and 1,2-DCB. Dechlorination involved multiple pathways including the removal of doubly flanked, singly flanked and isolated chlorine substituents. 454-pyrosequencing of partial bacterial 16S rRNA genes amplified from selected chlorobenzene dechlorinating sediment-free enrichment cultures revealed the presence of a variety of bacterial species, including Dehalobacter and Dehalococcoides mccartyi, that were previously documented as organohalide respiring bacteria. A genus with apparent close relationship to Desulfitobacterium that also has been associated with organohalide respiration, composed the major fraction of the operational taxonomic units (OTUs). Another major OTU was linked with Sedimentibacter sp., a genus that was previously identified in strict co-cultures of consortia reductively dehalogenating chlorinated compounds. Our data point towards the existence of multiple interactions within highly chlorinated benzene dechlorinating communities.
ISSN: 0923-9820
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Bioengineering Technology TC, Technology Campus De Nayer Sint-Katelijne-Waver
Division Soil and Water Management
Gynaecological Oncology
Technologiecluster Bioengineering Technologie
× corresponding author
# (joint) last author

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