Title: Model-based cell number quantification using online single-oxygen sensor data for tissue engineering perfusion bioreactors
Authors: Lambrechts, Toon
Papantoniou, Ioannis
Sonnaert, Maarten
Schrooten, Jan
Aerts, Jean-Marie # ×
Issue Date: Oct-2014
Publisher: Wiley
Series Title: Biotechnology and Bioengineering vol:111 issue:10 pages:1982-1992
Abstract: Online and non-invasive quantification of critical tissue engineering (TE) construct quality attributes in TE bioreactors is indispensable for the cost-effective up-scaling and automation of cellular construct manufacturing. However, appropriate monitoring techniques for cellular constructs in bioreactors are still lacking. This study presents a generic and robust approach to determine cell number and metabolic activity of cell-based TE constructs in perfusion bioreactors based on single oxygen sensor data in dynamic perfusion conditions. A data-based mechanistic modeling technique was used that is able to correlate the number of cells within the scaffold (R(2)  = 0.80) and the metabolic activity of the cells (R(2)  = 0.82) to the dynamics of the oxygen response to step changes in the perfusion rate. This generic non-destructive measurement technique is effective for a large range of cells, from as low as 1.0 × 10(5) cells to potentially multiple millions of cells, and can open-up new possibilities for effective bioprocess monitoring.
ISSN: 0006-3592
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division M3-BIORES: Measure, Model & Manage Bioresponses (-)
Skeletal Biology and Engineering Research Center (+)
Surface and Interface Engineered Materials
Department of Materials Engineering - miscellaneous
× corresponding author
# (joint) last author

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