Characterization of amino acids Arg, Ser and Thr at position 70 within HIV-1 reverse transcriptase

Megens, Sarah; De Wit, S; Bernatchez, J; Dekeersmaeker, N; Vinken, Lore; Covens, K; Theys, Kristof; Camacho, Ricardo Jorge; Vandamme, Anne-Mieke; Götte, M; Van Laethem, Kristel

doi:10.1179/2295333714Y.0000000038

Characterization of amino acids Arg, Ser and Thr at position 70 within HIV-1 reverse transcriptase

Author:

Megens, Sarah

De Wit, S ; Bernatchez, J ; Dekeersmaeker, N ; Vinken, Lore ; Covens, K ; Theys, Kristof ; Camacho, Ricardo Jorge ; Vandamme, Anne-Mieke ; Götte, M ; Van Laethem, Kristel

Keywords:

Science & Technology, Life Sciences & Biomedicine, Medicine, General & Internal, General & Internal Medicine, HIV-1, Resistance, Mutation, Reverse transcriptase, IMMUNODEFICIENCY-VIRUS TYPE-1, DRUG-RESISTANCE, IN-VITRO, MOLECULAR-MECHANISM, MUTATIONS, SUSCEPTIBILITY, ZIDOVUDINE, INHIBITORS, LAMIVUDINE, EMERGENCE, HIV-1,, Mutation,, Resistance,, Amino Acids, Bayes Theorem, Drug Resistance, Viral, HEK293 Cells, HIV Infections, HIV Reverse Transcriptase, Humans, Models, Molecular, 1101 Medical Biochemistry and Metabolomics, 3202 Clinical sciences

Abstract:

The amino acid position 70 in HIV-1 reverse transcriptase (RT) plays an important role in nucleoside RT inhibitor (NRTI) resistance. K70R is part of the thymidine analog mutations, but also other amino acid changes have been associated with NRTI resistance, such as K70E and K70G. In this study, we investigated the in vivo selection of the HIV-1 RT mutations K70S and K70T and their in vitro effect on drug resistance and replication capacity.