Title: Heterobifunctional PEG Ligands for Bioconjugation Reactions on Iron Oxide Nanoparticles
Authors: Bloemen, Maarten ×
Van Stappen, Thomas
Willot, Pieter
Lammertyn, Jeroen
Koeckelberghs, Guy
Geukens, Nick
Gils, Ann
Verbiest, Thierry #
Issue Date: 2-Oct-2014
Publisher: Public Library of Sciene
Series Title: PLoS One vol:9 issue:10
Article number: e109475
Abstract: Ever since iron oxide nanoparticles have been recognized as promising scaffolds for biomedical applications, their surface functionalization has become even more important. We report the synthesis of a novel polyethylene glycol-based ligand that combines multiple advantageous properties for these applications. The ligand is covalently bound to the surface via a siloxane group, while its polyethylene glycol backbone significantly improves the colloidal stability of the particle in complex environments. End-capping the molecule with a carboxylic acid introduces a variety of coupling chemistry possibilities. In this study an antibody targeting plasminogen activator inhibitor-1 was coupled to the surface and its presence and binding activity was assessed by enzyme-linked immunosorbent assay and surface plasmon resonance experiments. The results indicate that the ligand has high potential towards biomedical applications where colloidal stability and advanced functionality is crucial.
ISSN: 1932-6203
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Imaging and Photonics
Therapeutic and Diagnostic Antibodies (+)
Polymer Chemistry and Materials
Division of Mechatronics, Biostatistics and Sensors (MeBioS)
Chemistry, Campus Kulak Kortrijk
Chemistry - miscellaneous
× corresponding author
# (joint) last author

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