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Title: Phenotypic evaluation of natural and industrial Saccharomyces yeasts for different traits desirable in industrial bioethanol production
Authors: Mukherjee, Vaskar * ×
Steensels, Jan *
Lievens, Bart
Van De Voorde, Ilse
Verplaetse, Alex
Aerts, Guido
Willems, Kris
Thevelein, Johan
Verstrepen, Kevin
Ruyters, Stefan #
Issue Date: Nov-2014
Publisher: Springer-Verlag
Series Title: Applied Microbiology and Biotechnology vol:98 issue:22 pages:9483-98
Abstract: Saccharomyces cerevisiae is the organism of choice for many food and beverage fermentations because it thrives in high-sugar and high-ethanol conditions. However, the conditions encountered in bioethanol fermentation pose specific challenges, including extremely high sugar and ethanol concentrations, high temperature, and the presence of specific toxic compounds. It is generally considered that exploring the natural biodiversity of Saccharomyces strains may be an interesting route to find superior bioethanol strains and may also improve our understanding of the challenges faced by yeast cells during bioethanol fermentation. In this study, we phenotypically evaluated a large collection of diverse Saccharomyces strains on six selective traits relevant for bioethanol production with increasing stress intensity. Our results demonstrate a remarkably large phenotypic diversity among different Saccharomyces species and among S. cerevisiae strains from different origins. Currently applied bioethanol strains showed a high tolerance to many of these relevant traits, but several other natural and industrial S. cerevisiae strains outcompeted the bioethanol strains for specific traits. These multitolerant strains performed well in fermentation experiments mimicking industrial bioethanol production. Together, our results illustrate the potential of phenotyping the natural biodiversity of yeasts to find superior industrial strains that may be used in bioethanol production or can be used as a basis for further strain improvement through genetic engineering, experimental evolution, or breeding. Additionally, our study provides a basis for new insights into the relationships between tolerance to different stressors.
ISSN: 0175-7598
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Bioengineering Technology TC, Technology Campus De Nayer Sint-Katelijne-Waver
Centre of Microbial and Plant Genetics
Technologiecluster Bioengineering Technologie
Bioengineering Technology TC, Technology Campuses Ghent and Aalst
Molecular Microbiology and Biotechnology Section - miscellaneous
* (joint) first author
× corresponding author
# (joint) last author

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Mukherjee et al. 2014 OA.pdfOA article Accepted 1558KbAdobe PDFView/Open
Supplementary material final.pdfSupplementary material Accepted 1897KbAdobe PDFView/Open

 


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