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Title: Heterogeneity and phenotypic plasticity of glial cells in the mammalian enteric nervous system
Authors: Boesmans, Werend *
Lasrado, Reena *
Vanden Berghe, Pieter #
Pachnis, Vassilis # ×
Issue Date: Feb-2015
Publisher: Wiley-Liss, Inc.
Series Title: GLIA vol:63 issue:2 pages:229-241
Article number: 10.1002/glia.22746
Abstract: Enteric glial cells are vital for the autonomic control of gastrointestinal homeostasis by the enteric nervous system. Several different functions have been assigned to enteric glial cells but whether these are performed by specialized subtypes with a distinctive phenotype and function remains elusive. We used Mosaic Analysis with Double Markers and inducible lineage tracing to characterize the morphology and dynamic molecular marker expression of enteric GLIA in the myenteric plexus. Functional analysis in individually identified enteric glia was performed by Ca(2+) imaging. Our experiments have identified four morphologically distinct subpopulations of enteric glia in the gastrointestinal tract of adult mice. Marker expression analysis showed that the majority of glia in the myenteric plexus co-express glial fibrillary acidic protein (GFAP), S100β, and Sox10. However, a considerable fraction (up to 80%) of glia outside the myenteric ganglia, did not label for these markers. Lineage tracing experiments suggest that these alternative combinations of markers reflect dynamic gene regulation rather than lineage restrictions. At the functional level, the three myenteric glia subtypes can be distinguished by their differential response to adenosine triphosphate. Together, our studies reveal extensive heterogeneity and phenotypic plasticity of enteric glial cells and set a framework for further investigations aimed at deciphering their role in digestive function and disease. GLIA 2014 Main points: Enteric glia can be discriminated on the basis of morphology and location but also display distinct molecular and functional characteristics. The diversity among enteric glia reflects dynamic gene regulation rather than lineage restrictions.
URI: 
ISSN: 0894-1491
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Translational Research in GastroIntestinal Disorders
* (joint) first author
× corresponding author
# (joint) last author

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