Title: The skin microbiome of caspase-14-deficient mice shows mild dysbiosis
Authors: Kubica, Malgorzata ×
Hildebrand, Falk
Brinkman, Brigitta M
Goossens, Dirk
Del Favero, Jurgen
Vercammen, Ken
Cornelis, Pierre
Schroeder, Jens-Michael
Vandenabeele, Peter
Raes, Jeroen
Declercq, Wim #
Issue Date: Aug-2014
Publisher: Munksgaard
Series Title: Experimental Dermatology vol:23 issue:8 pages:561-567
Abstract: Caspase-14, an important proteinase involved in filaggrin catabolism, is mainly active in terminally differentiating keratinocytes, where it is required for the generation of skin natural moisturizing factors (NMFs). Consequently, caspase-14 deficient epidermis is characterized by reduced levels of NMFs such as urocanic acid and 2-pyrrolidone-5-carboxylic acid. Patients suffering from filaggrin deficiency are prone to develop atopic dermatitis, which is accompanied with increased microbial burden. Among several reasons, this effect could be due to a decrease in filaggrin breakdown products. In this study, we found that caspase-14(-/-) mice show enhanced antibacterial response compared to wild-type mice when challenged with bacteria. Therefore, we compared the microbial communities between wild-type and caspase-14(-/-) mice by sequencing of bacterial 16S ribosomal RNA genes. We observed that caspase-14 ablation leads to an increase in bacterial richness and diversity during steady-state conditions. Although both wild-type and caspase-14(-/-) skin were dominated by the Firmicutes phylum, the Staphylococcaceae family was reduced in caspase-14(-/-) mice. Altogether, our data demonstrated that caspase-14 deficiency causes the imbalance of the skin-resident bacterial communities.
ISSN: 0906-6705
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Department of Microbiology & Immunology - miscellaneous
Division of Geography & Tourism
Laboratory of Molecular Bacteriology (Rega Institute)
× corresponding author
# (joint) last author

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