Title: Transcriptional diversity during lineage commitment of human blood progenitors
Authors: Chen, Lu ×
Kostadima, Myrto
Martens, Joost H A
Canu, Giovanni
Garcia, Sara P
Turro, Ernest
Downes, Kate
Macaulay, Iain C
Bielczyk-Maczynska, Ewa
Coe, Sophia
Farrow, Samantha
Poudel, Pawan
Burden, Frances
Jansen, Sjoert B G
Astle, William J
Attwood, Antony
Bariana, Tadbir
de Bono, Bernard
Breschi, Alessandra
Chambers, John C
BRIDGE Consortium
Choudry, Fizzah A
Clarke, Laura
Coupland, Paul
van der Ent, Martijn
Erber, Wendy N
Jansen, Joop H
Favier, Rémi
Fenech, Matthew E
Foad, Nicola
Freson, Kathleen
Van Geet, Chris
Gomez, Keith
Guigo, Roderic
Hampshire, Daniel
Kelly, Anne M
Kerstens, Hindrik H D
Kooner, Jaspal S
Laffan, Michael
Lentaigne, Claire
Labalette, Charlotte
Martin, Tiphaine
Meacham, Stuart
Mumford, Andrew
Nürnberg, Sylvia
Palumbo, Emilio
van der Reijden, Bert A
Richardson, David
Sammut, Stephen J
Slodkowicz, Greg
Tamuri, Asif U
Vasquez, Louella
Voss, Katrin
Watt, Stephen
Westbury, Sarah
Flicek, Paul
Loos, Remco
Goldman, Nick
Bertone, Paul
Read, Randy J
Richardson, Sylvia
Cvejic, Ana
Soranzo, Nicole
Ouwehand, Willem H
Stunnenberg, Hendrik G
Frontini, Mattia
Rendon, Augusto #
Issue Date: Sep-2014
Publisher: American Association for the Advancement of Science
Series Title: Science vol:345 issue:6204
Article number: 1251033
Abstract: Blood cells derive from hematopoietic stem cells through stepwise fating events. To characterize gene expression programs driving lineage choice, we sequenced RNA from eight primary human hematopoietic progenitor populations representing the major myeloid commitment stages and the main lymphoid stage. We identified extensive cell type-specific expression changes: 6711 genes and 10,724 transcripts, enriched in non-protein-coding elements at early stages of differentiation. In addition, we found 7881 novel splice junctions and 2301 differentially used alternative splicing events, enriched in genes involved in regulatory processes. We demonstrated experimentally cell-specific isoform usage, identifying nuclear factor I/B (NFIB) as a regulator of megakaryocyte maturation-the platelet precursor. Our data highlight the complexity of fating events in closely related progenitor populations, the understanding of which is essential for the advancement of transplantation and regenerative medicine.
ISSN: 0036-8075
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular and Vascular Biology
× corresponding author
# (joint) last author

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