Title: Importance of biofilm formation and dipeptidyl peptidase IV for the pathogenicity of clinical Porphyromonas gingivalis isolates
Authors: Clais, Sofie ×
Boulet, Gaëlle
Kerstens, Monique
Horemans, Tessa
Teughels, Wim
Quirynen, Marc
Lanckacker, Ellen
De Meester, Ingrid
Lambeir, Anne-Marie
Delputte, Peter
Maes, Louis
Cos, Paul #
Issue Date: Apr-2014
Series Title: Pathogens and disease vol:70 issue:3 pages:408-13
Article number: 10.1111/2049-632X.12156
Abstract: The ability of Porphyromonas gingivalis to cause adult periodontitis is determined by its arsenal of virulence factors. Here, we investigated the importance of biofilm formation and bacterial dipeptidyl peptidase IV (DPPIV) for the pathogenicity of clinical P. gingivalis isolates. In our study, the isolates with biofilm-forming capacity also showed high DPPIV activity in vitro. Moreover, DPPIV activity increased in P. gingivalis biofilms compared to planktonic cells. In a murine subcutaneous abscess model, the biofilm-forming isolates with high DPPIV activity proved to be pathogenic, while the nonbiofilm formers with low DPPIV activity did not induce abscesses. The biofilm-forming ATCC 33277 strain with low DPPIV activity was not pathogenic in mice either. Our results suggest that biofilm formation and DPPIV activity contribute to the pathogenic potential of P. gingivalis. Furthermore, we show that biofilm formation may enhance P. gingivalis virulence through an increased DPPIV activity. Because of their importance for bacterial colonization and growth, biofilm formation and DPPIV activity could present interesting therapeutic targets to tackle periodontitis.
ISSN: 2049-632X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Periodontology
× corresponding author
# (joint) last author

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