Title: Differential peptidomics highlights adipokinetic hormone as key player in regulating digestion in anautogenous flesh fly, Sarcophaga crassipalpis
Authors: Bil, Magdalena ×
Broeckx, Valerie
Landuyt, Bart
Huybrechts, Roger #
Issue Date: 1-Nov-2014
Publisher: Academic Press
Series Title: General and Comparative Endocrinology vol:208 pages:49-56
Abstract: Females of anautogenous flesh flies, Sarcophaga crassipalpis, need a protein meal in order to
produce their first batch of eggs. This protein meal elicits an increase in midgut proteolytic activity that
is under neuropeptidergic regulation. Time series of decapitation and rescue experiments of liver fed
flies evidenced the need of a peptide factor released by corpora cardiaca (CC) within four hours post
protein feeding in order to assure complete protein digestion. Q-Exactive quantitative differential
peptidomics analysis on CC of sugar fed flies and flies five hours post protein feeding respectively,
showed a unique consistent decrease in the stored amount of adipokinetic hormone (AKH) ranging
between 16 up to 63%. Injection of AKH into liver fed decapitated flies as well as sugar fed intact flies
resulted in dose dependent enhanced midgut proteolytic activity up to the level of intact protein fed
flies. This suggests a key role of AKH in food depended reproduction
ISSN: 0016-6480
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Animal Physiology and Neurobiology Section - miscellaneous
× corresponding author
# (joint) last author

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