Title: Spatio-temporal dynamics of fructan metabolism in developing barley grains
Authors: Peukert, Manuela ×
Thiel, Johannes
Peshev, Darin
Weschke, Winfriede
Van den Ende, Wim
Mock, Hans-Peter
Matros, Andrea #
Issue Date: Sep-2014
Publisher: American Society of Plant Physiologists
Series Title: The Plant Cell vol:26 pages:3728-3744
Abstract: Barley (Hordeum vulgare) grain development follows a series of defined morphological and physiological stages and depends on the supply of assimilates (mainly sucrose) from the mother plant. Here, spatio-temporal patterns of sugar distributions were investigated by mass spectrometric imaging, targeted metabolite analyses, and transcript profi
ling of microdissected grain tissues. Distinct spatio-temporal sugar balances were observed, which may relate to differentiation and grain filling processes. Notably, various types of oligofructans showed specific distribution patterns. Levan- and graminan-type oligofructans were synthesized in the cellularized endosperm prior to the commencement of starch biosynthesis, while during
the storage phase, inulin-type oligofructans accumulated to a high concentration in and around the nascent endosperm
cavity. In the shrunken endosperm mutant seg8 , with a decreased sucrose flux toward the endosperm, fructan accumulation was impaired. The tight partitioning of oligofructan biosynthesis hints at distinct functions of the various fructan types in the young endosperm prior to starch accumulation and in the endosperm transfer cells that accomplish the assimilate supply toward the endosperm at the storage phase.
ISSN: 1040-4651
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Physiology of Plants and Micro-organisms Section - miscellaneous
× corresponding author
# (joint) last author

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