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Title: A cost-effective approach to microporate mammalian cells with the Neon Transfection System
Authors: Brees, Chantal
Fransen, Marc # ×
Issue Date: 1-Dec-2014
Publisher: Elsevier
Series Title: Analytical Biochemistry vol:466 pages:49-50
Article number: S0003-2697(14)00350-9
Abstract: Electroporation is one of the most efficient non-viral methods for transferring exogenous DNA into mammalian cells. However, the relatively high costs of electroporation kits and reagents temper the routine use of this fast and easy to perform technique in many laboratories. Several years ago, a new flexible and easy to operate electroporation device was launched under the name "Neon(TM) Transfection System". This device uses specialized pipette tips containing gold-plated electrodes as electroporation chamber. Here we report a protocol to regenerate these expensive tips as well as some other Neon(TM) kit accessories, thereby reducing the cost of electroporation at least ten-fold.
ISSN: 0003-2697
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Lipid Biochemistry and Protein Interactions
× corresponding author
# (joint) last author

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