Title: Unprocessed barley aleurone endo-beta-1,4-xylanase X-I is an active enzyme
Authors: Van Campenhout, Steven ×
Pollet, Annick
Bourgois, Tine
Rombouts, Sigrid
Beaugrand, Johnny
Gebruers, Kurt
De Backer, Evelien
Courtin, Christophe
Delcour, Jan
Volckaert, Guido #
Issue Date: 11-Apr-2007
Series Title: Biochemical and Biophysical Research Communications vol:356 issue:3 pages:799-804
Abstract: Endo-beta-1,4-xylanase X-I is a major hydrolase produced by the aleurone tissue of germinating barley grain. It was previously reported that this cytosolic enzyme is synthesized as an inactive precursor which is proteolytically processed to active forms upon its programmed cell death dependent release. We here demonstrate, however, that the precursor form of X-I is an active enzyme. Purified recombinant precursor X-I was characterised with respect to its molecular weight, iso-electric point and temperature and pH activity and stability. Analysis of the hydrolysis products showed that it is an endo-acting enzyme which has the striking ability to release xylose from both polymeric xylan as well as from small xylo-oligosaccharides. The implications of these findings in relation to the putative role of the N-terminal propeptide as a carbohydrate binding module and the possible consequences for the way X-I fulfils its role in the germination process, are discussed.
ISSN: 0006-291X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre for Food and Microbial Technology
Division of Gene Technology (-)
× corresponding author
# (joint) last author

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