Title: Metabolite profiling and peptidoglycan analysis of transient cell wall-deficient bacteria in a new Escherichia coli model system
Authors: Cambré, Alexander
Zimmerman, Michael
Sauer, Uwe
Vivijs, Bram
Cenens, William
Michiels, Chris W.
Aertsen, Abram
Loessner, Martin J
Noben, Jean-Paul
Ayala, Juan A
Lavigne, Rob #
Briers, Yves # ×
Issue Date: May-2015
Publisher: Blackwell Science
Series Title: Environmental Microbiology vol:17 issue:5 pages:1586-1599
Abstract: Many bacteria are able to assume a transient cell wall-deficient (or L-form) state under favorable osmotic conditions. Cell wall stress such as exposure to β-lactam antibiotics can enforce the transition to and maintenance of this state. L-forms actively proliferate and can return to the walled state upon removal of the inducing agent. We have adopted Escherichia coli as a model system for the controlled transition to and reversion from the L-form state, and have studied these dynamics with genetics, cell biology and 'omics' technologies. As such, a transposon mutagenesis screen underscored the requirement for the Rcs phosphorelay and colanic acid synthesis, while proteomics show only little differences between rods and L-forms. In contrast, metabolome comparison reveals the high abundance of lysophospholipids and phospholipids with unsaturated or cyclopropanized fatty acids in E. coli L-forms. This increase of membrane lipids associated with increased membrane fluidity may facilitate proliferation through bud formation. Visualization of the residual peptidoglycan with a fluorescently labeled peptidoglycan binding protein indicates de novo cell wall synthesis and a role for septal peptidoglycan synthesis during bud constriction. The DD-carboxypeptidases PBP5 and PBP6 are three- and four-fold upregulated in L-forms, indicating a specific role for regulation of crosslinking during L-form proliferation.
ISSN: 1462-2912
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Gene Technology (-)
Centre for Food and Microbial Technology
× corresponding author
# (joint) last author

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