Title: Interaction between penicillin and the DD-carboxypeptidase of the unstable L-form of Proteus mirabilis strain 19
Authors: Schilf, W ×
Frère, P
Frère, J M
Martin, H H
Ghuysen, J M
Adriaens, Paul
Meesschaert, Boudewijn #
Issue Date: 1978
Publisher: Springer verlag
Series Title: European Journal of Biochemistry vol:85 issue:2 pages:325-30
Abstract: Binding of penicillin to the DD-carboxypeptidase of the unstable spheroplast L-form of Proteus mirabilis results in the rapid formation of a modified enzyme-inhibitor complex which in turn undergoes rapid decay into reactivated enzyme and an antibiotically inactive penicillin degradation product. Major antibiotic metabolites recovered from such interactions were benzylpenicilloic acid and phenoxymethylpenicilloic acid from benzylpenicillin and phenoxymethylpenicillin, respectively, suggesting a second enzymic function of the DD-carboxypeptidase as a penicillinase of low efficiency. Statistical analyses made with the help of a linear regression program show that the enzyme interacts with the substrate UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-(L)-meso-2,6-diaminopimelyl-(L)-D-alanyl-D-alanine and either benzympenicillin or carbenicillin in a non-competitive manner.
ISSN: 0014-2956
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Radiology
Microbial and Molecular Systems - miscellaneous
Technologiecluster Bioengineering Technologie
Bioengineering Technology TC, Campus Bruges
× corresponding author
# (joint) last author

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