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Title: Studies on the biosynthesis of isopenicillin N with a cell-free preparation of Penicillium chrysogenum
Authors: Meesschaert, B ×
Adriaens, Paul
Eyssen, Hendrik #
Issue Date: Jun-1980
Series Title: The Journal of antibiotics vol:33 issue:7 pages:722-30
Abstract: When delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine was added to a cell-free system prepared by lysis of Penicillium chrysogenum protoplasts, "compounds X and Y" were detected after analysis on a cation-exchange column. The chromatographic position as well as results of experiments with double labelled tripeptides showed "compound X" to be the penicilloic acid of isopenicillin N. LLD-Tripeptide labelled with tritium at carbon-2 of th valine part was incorporated into isopenicillin N with retention of label. "Compound Y" retained all hydrogens on the valine part of the peptide, but lost half of the tritium on carbon-3 of the cysteine part. The results are consistent with the hypothesis that the LLD-tripeptide is converted into isopenicillin N via a monocyclic beta-lactam and without a dehydrovalinyl intermediate. Extensive transacylase activity was observed between isopenicillin N and 6-aminopenicillanic acid.
ISSN: 0021-8820
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Radiology
× corresponding author
# (joint) last author

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