Title: A wheat 1-FEH w3 variant underlies enzyme activity for stem WSC remobilization to grain under drought
Authors: Zhang, Jingjuan
Xu, Yunji
Chen, Wei
Dell, Bernard
Vergauwen, Rudy
Biddulph, Ben
Khan, Nusrat
Luo, Hao
Appels, Rudi
Van den Ende, Wim # ×
Issue Date: Jan-2015
Publisher: Wiley-Blackwell Publishing Ltd.
Series Title: New Phytologist vol:205 issue:1 pages:293-305
Abstract: • In wheat stems, levels of fructan-dominated water soluble carbohydrates (WSC) do not always correlate well with grain yield.
• Field experiments were carried out to further elucidate this relationship. Westonia, Kauz and ~20 genetically diverse double haploid (DH) lines derived from them were investigated.
• Substantial genotypic differences in fructan remobilization were found.
• The results of enzyme activity and gene expression studies confirmed the major contribution of 1-FEH w3 in the process of stem fructan remobilization.
• A Single Nucleotide Polymorphism (SNP) was detected in an auxin response element in the promoter region of 1-FEH w3 and the mutated Westonia allele might affect gene expression and enzyme activity levels.
• The mutated Westonia allele of 1-FEH w3 was associated with a higher thousand grain weight (TGW) under drought in 2011 and 2012.
• A Cleaved Amplified Polymorphic (CAP) marker was generated from the SNP.
• These results indicate that higher gene expression of 1-FEH w3 and 1-FEH w3 mediated enzyme activities favour stem WSC remobilization to the grains. The CAP marker residing in the 1-FEH w3 promoter may facilitate wheat breeding, by selecting lines with high stem fructan remobilization capacity under terminal drought.
ISSN: 0028-646X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Physiology of Plants and Micro-organisms Section - miscellaneous
× corresponding author
# (joint) last author

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