Title: Electrochemical sensor with dry reagents implemented in lab-on-chip for single nucleotide polymorphism detection
Authors: Tanaka, Hirohuki ×
Fiorini, Paolo
Jones, Ben
Peeters, Sara
Wiederkehr, Rodrigo Sergio
Majeed, Bivragh
Yaku, Hidenobu
Hiraoka, M
Hiraoka, Maki
Matsuno, Toshinobu
Yamashita, Ichiro #
Issue Date: Apr-2014
Publisher: Publication Board, Japanese Journal of Applied Physics
Series Title: Japanese Journal of Applied Physics 1, Regular Papers, Short Notes & Review Papers vol:53 issue:5
Article number: 05FS03
Abstract: We developed an electrochemical (EC) sensor having dry reagents to detect pyrophosphoric acid (PPi) produced as a by-product of a polymerasechain-
reaction (PCR) amplicon for single nucleotide polymorphism (SNP) detection. The EC sensor is implementable in a lab-on-chip (LoC)
system, and a sensor chip having side-wall electrical connections that enable electrical contacts from the top of the LoC has been developed. We
also developed separated on-chip placement of dry reagents divided into three groups in a sensor cavity to suppress background current when
there is no PPi. Using this chip, we successfully demonstrated SNP detection in the ABO gene from human blood samples, in combination with the
allele-specific PCR amplification method using our developed LoC system
ISSN: 0021-4922
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

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