Author:

Keywords:

Science & Technology, Life Sciences & Biomedicine, Cell & Tissue Engineering, Biotechnology & Applied Microbiology, Oncology, Cell Biology, Hematology, Hematopoietic stem cells, Niche, Mobilization, Homing, CXCL12, SMAD signaling, Osteoblasts, STEM-CELL NICHE, COLONY-STIMULATING FACTOR, SHORT INTERFERING RNAS, MORPHOGENETIC PROTEIN-4, PERIPHERAL-BLOOD, G-CSF, CHEMOKINE SDF-1, MICE, MAINTENANCE, RECEPTOR, Animals, Bone Marrow, Bone Marrow Cells, Bone Morphogenetic Protein 4, Cell Lineage, Cell Movement, Cells, Cultured, Chemokine CXCL12, Gene Expression Regulation, Hematopoietic Stem Cell Transplantation, Hematopoietic Stem Cells, Mice, Receptors, CXCR4, Signal Transduction, Smad Proteins, Stem Cell Niche, 06 Biological Sciences, 10 Technology, 11 Medical and Health Sciences, Immunology, 31 Biological sciences, 32 Biomedical and clinical sciences

Abstract:

We recently demonstrated that ex vivo activation of SMAD-independent bone morphogenetic protein 4 (BMP4) signaling in hematopoietic stem/progenitor cells (HSPCs) influences their homing into the bone marrow (BM). Here, we assessed whether alterations in BMP signaling in vivo affects adult hematopoiesis by affecting the BM niche. We demonstrate that systemic inhibition of SMAD-dependent BMP signaling by infusion of the BMP antagonist noggin (NGN) significantly increased CXCL12 levels in BM plasma leading to enhanced homing and engraftment of transplanted HSPCs. Conversely, the infusion of BMP7 but not BMP4, resulted in decreased HSPC homing. Using ST2 cells as an in vitro model of BM niche, we found that incubation with neutralizing anti-BMP4 antibodies, NGN, or dorsomorphin (DM) as well as knockdown of Smad1/5 and Bmp4, all enhanced CXCL12 production. Chromatin immunoprecipitation identified the SMAD-binding element in the CXCL12 promoter to which SMAD4 binds. When deleted, increased CXCL12 promoter activity was observed, and NGN or DM no longer affected Cxcl12 expression. Interestingly, BMP7 infusion resulted in mobilization of only short-term HSCs, likely because BMP7 affected CXCL12 expression only in osteoblasts but not in other niche components. Hence, we describe SMAD-dependent BMP signaling as a novel regulator of CXCL12 production in the BM niche, influencing HSPC homing, engraftment, and mobilization.