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Title: Spatial optimization in perfusion bioreactors improves bone tissue-engineered construct quality attributes
Authors: Papantoniou, Ioannis ×
Guyot, Yann
Sonnaert, Maarten
Kerckhofs, Greet
Luyten, Frank
Geris, Liesbet
Schrooten, Jan #
Issue Date: Dec-2014
Publisher: Wiley
Series Title: Biotechnology and Bioengineering vol:111 issue:12 pages:2560-70
Article number: 10.1002/bit.25303
Abstract: Perfusion bioreactors have shown great promise for tissue engineering applications providing a homogeneous and consistent distribution of nutrients and flow-induced shear stresses throughout tissue-engineered constructs. However, non uniform fluid-flow profiles found in the perfusion chamber entrance region have been shown to affect tissue-engineered construct quality characteristics during culture. In this study a whole perfusion and construct, three dimensional (3D) computational fluid dynamics approach was used in order to optimize a critical design parameter such as the location of the regular pore scaffolds within the perfusion bioreactor chamber. Computational studies were coupled to bioreactor experiments for a case-study flow rate. Two cases were compared in the first instance seeded scaffolds were positioned immediately after the perfusion chamber inlet while a second group was positioned at the computationally determined optimum distance were a steady state flow profile had been reached. Experimental data showed that scaffold location affected significantly cell content and neo-tissue distribution, as determined and quantified by contrast enhanced nanoCT, within the constructs both at 14 and 21 days of culture. However gene expression level of osteopontin and osteocalcin was not affected by the scaffold location. This study demonstrates that the bioreactor chamber environment, incorporating a scaffold and its location within it, affects the flow patterns within the pores throughout the scaffold requiring therefore dedicated optimization that can lead to bone tissue engineered constructs with improved quality attributes. Biotechnol. Bioeng. © 2014 Wiley Periodicals, Inc.
URI: 
ISSN: 0006-3592
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Skeletal Biology and Engineering Research Center (+)
Biomechanics Section
Surface and Interface Engineered Materials
Structural Composites and Alloys, Integrity and Nondestructive Testing
Department of Materials Engineering - miscellaneous
× corresponding author
# (joint) last author

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