Title: Derivatives of the mouse cathelicidin-related antimicrobial peptide (CRAMP) inhibit fungal and bacterial biofilm formation
Authors: De Brucker, Katrijn *
Delattin, Nicolas *
Robijns, Stijn
Steenackers, Hans
Verstraeten, Natalie
Landuyt, Bart
Luyten, Walter
Schoofs, Liliane
Dovgan, B.
Fröhlich, M.
Michiels, Jan
Vanderleyden, Jos
Cammue, Bruno ×
Thevissen, Karin #
Issue Date: Sep-2014
Publisher: American Society for Microbiology (ASM)
Series Title: Antimicrobial Agents and Chemotherapy vol:58 issue:9 pages:5395-5404
Abstract: We identified a 26-amino acid truncated form of the 34-amino acid cathelicidin-related antimicrobial peptide (CRAMP) in the islets of Langerhans of the murine pancreas. This peptide, P318, shares 67% identity with the human antimicrobial peptide LL-37. As LL-37 displays antimicrobial and antibiofilm activity, we tested antifungal and antibiofilm activity of P318 against the fungal pathogen Candida albicans. P318 shows biofilm-specific activity as it inhibits C. albicans biofilm formation at 0.15 µM without affecting planktonic survival at these concentrations. Next, we tested the C. albicans biofilm inhibitory activity of a series of truncated and alanine-substituted derivatives of P318. Based on the biofilm inhibitory activity of these derivatives and the length of the peptides, we decided to synthesize the shortened alanine-substituted peptide at position 10 (AS10, KLKKIAQKIKNFFQKLVP). AS10 inhibited C. albicans biofilm formation at 0.22 µM, and acted synergistically with amphotericin B and caspofungin against mature biofilms. AS10 also inhibited biofilm formation of different bacteria as well as of fungi and bacteria in a mixed biofilm. In addition, AS10 does not affect viability or functionality of different cell types involved in osseointegration of an implant, pointing to the potential of AS10 as a lead peptide for further development to coat implants.
ISSN: 0066-4804
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre of Microbial and Plant Genetics
Animal Physiology and Neurobiology Section - miscellaneous
Department of Pharmaceutical & Pharmacological Sciences - miscellaneous
* (joint) first author
× corresponding author
# (joint) last author

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