Title: Instability of bacteriophages in spray-dried trehalose powders is caused by crystallization of the matrix
Authors: Vandenheuvel, Dieter
Meeus, Joke
Lavigne, Rob #
Van den Mooter, Guy # ×
Issue Date: Sep-2014
Publisher: Elsevier/North Holland
Series Title: International Journal of Pharmaceutics vol:472 issue:1-2 pages:202-5
Article number: S0378-5173(14)00446-3
Abstract: Spray drying is a valuable technique in pharmaceutical dosage formulation, capable of producing amorphous, spherical powders, suitable for pulmonary deposition and further downstream processing. In this study, we show that spray drying bacteriophages together with trehalose results in an amorphous powder matrix with high glass transition temperature (between 116 and 118°C), typical for amorphous trehalose. These powders are stable at low temperatures (4°C) and relative humidity (0%). However, high humidity causes crystallization of the amorphous matrix, destroying the embedded phages. Furthermore, storage at higher temperature (25°C) causes thermal instability of the embedded phages. The results show that storage conditions are important parameters to take into account in phage therapy development. The resulting particles are hollow spheres, with suitable aerodynamic diameters for deposition into the deep lungs. This opens possibilities to use these phage-containing powder formulations to tackle pulmonary infectious diseases, especially caused by antibiotic resistant pathogens.
ISSN: 0378-5173
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Gene Technology (-)
Drug Delivery and Disposition
× corresponding author
# (joint) last author

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