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Title: Syntenin-1 and ezrin proteins link activated leukocyte cell adhesion molecule to the actin cytoskeleton
Authors: Tudor, Cicerone *
Te Riet, Joost *
Eich, Christina
Harkes, Rolf
Smisdom, Nick
Bouhuijzen Wenger, Jessica
Ameloot, Marcel
Holt, Matthew
Kanger, Johannes S
Figdor, Carl G
Cambi, Alessandra
Subramaniam, Vinod # ×
Issue Date: May-2014
Publisher: American Society for Biochemistry and Molecular Biology
Series Title: Journal of Biological Chemistry vol:289 issue:19 pages:13445-60
Article number: 10.1074/jbc.M113.546754
Abstract: Activated leukocyte cell adhesion molecule (ALCAM) is a type I transmembrane protein member of the immunoglobulin superfamily of cell adhesion molecules. Involved in important pathophysiological processes such as the immune response, cancer metastasis, and neuronal development, ALCAM undergoes both homotypic interactions with other ALCAM molecules and heterotypic interactions with the surface receptor CD6 expressed at the T cell surface. Despite biochemical and biophysical evidence of a dynamic association between ALCAM and the actin cytoskeleton, no detailed information is available about how this association occurs at the molecular level. Here, we exploit a combination of complementary microscopy techniques, including FRET detected by fluorescence lifetime imaging microscopy and single-cell force spectroscopy, and we demonstrate the existence of a preformed ligand-independent supramolecular complex where ALCAM stably interacts with actin by binding to syntenin-1 and ezrin. Interaction with the ligand CD6 further enhances these multiple interactions. Altogether, our results propose a novel biophysical framework to understand the stabilizing role of the ALCAM supramolecular complex engaged to CD6 during dendritic cell-T cell interactions and provide novel information on the molecular players involved in the formation and signaling of the immunological synapse at the dendritic cell side.
ISSN: 0021-9258
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Department of Human Genetics - miscellaneous
Laboratory of Glia Biology (+)
Biomedical Sciences Group
* (joint) first author
× corresponding author
# (joint) last author

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