Title: pcSOFI as a Smart Label-Based Superresolution Microscopy Technique
Authors: Moeyaert, Benjamien
Dedecker, Peter # ×
Issue Date: 2014
Publisher: Humana Press, Inc.
Series Title: Methods in Molecular Biology vol:1148 pages:261-76
Article number: 10.1007/978-1-4939-0470-9_17
Abstract: Stochastic optical fluctuation imaging (SOFI) is a superresolution imaging technique that uses the flickering of fluorescent labels to generate a microscopic image with a resolution better than what the diffraction limit allows. Its adaptation towards fluorescent protein-labeled samples (called photoconversion SOFI or pcSOFI) allows for a straightforward and easily accessible way of generating superresolution images. In this protocol, we will discuss how so-called "smart labels," and specifically the reversibly switchable fluorescent proteins, have opened doors towards superresolution imaging in general and we provide a protocol on how to perform pcSOFI on HeLa cells expressing human β-actin labeled with the reversibly photoswitchable fluorescent protein Dronpa.
ISSN: 1064-3745
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Imaging and Photonics
× corresponding author
# (joint) last author

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