Title: Differential gene expression in Azospirillum spp. by plant root exudates: analysis of protein profiles by two-dimensional polyacrylamide gel electrophoresis
Authors: Van Bastelaere, E ×
De Mot, René
Michiels, Christiaan
Vanderleyden, Jozef #
Issue Date: 1993
Publisher: Elsevier Science BV
Series Title: FEMS Microbiology Letters vol:112 pages:335-342
Abstract: Two-dimensional polyacrylamide gel electrophoresis of total proteins of Azospirillum brasilense Sp7 showed that the bacteria respond to the presence of plant root exudates by induction of some proteins. High-level induction of a 40-kDa acidic protein was demonstrated by Coomassie brilliant blue staining and Western blotting, using a specific polyclonal antiserum. This protein is also observed in A. lipoferum, but not in A. halopraeferens and A. irakense. In A. brasilense, the 40-kDa protein was induced by exudates from wheat, maize, bean and alfalfa. However, only maize exudates stimulated production of this protein in A. lipoferum.
ISSN: 0378-1097
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre of Microbial and Plant Genetics
Centre for Food and Microbial Technology
× corresponding author
# (joint) last author

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