Title: Isolation and characterization of a pSym locus of Rhizobium sp BR816 that extends nodulation ability of narrow host range Phaseolus vulgaris symbionts to Leucaena leucocephala
Authors: Van Rhijn, Petronella ×
Luyten, Ellen
Vlassak, Katrien
Vanderleyden, Jozef #
Issue Date: Jan-1996
Publisher: APS Press
Series Title: Molecular Plant - Microbe Interactions vol:9 issue:1 pages:74-77
Abstract: Introduction of a cosmid library of megaplasmid DNA of Rhizobium sp. BR816, a broad host range Rhizobium strain, into R, etli CE3, a narrow host range bean symbiont, resulted in the isolation of a transconjugant that could effectively nodulate Leucaena leucocephala, Analysis of the corresponding cosmid, pBRF2, revealed the presence of genes required for eliciting nitrogen-fixing nodules on L. leucocephala. Subcloning and Tn5 tagging identified a locus responsible for the host range extension, Sequence analysis of this locus revealed an ORF that shows significant identity with NodO of R, leguminosarum by. vicine.
ISSN: 0894-0282
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre of Microbial and Plant Genetics
× corresponding author
# (joint) last author

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