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Title: Improved linkage analysis of Quantitative Trait Loci using bulk segregants unveils a novel determinant of high ethanol tolerance in yeast
Authors: Duitama, Jorge ×
Sanchez Rodriguez, Aminael
Goovaerts, Annelies
Pulido Tamayo, Sergio
Hubmann, Georg
Foulquié-Moreno, María R
Thevelein, Johan
Verstrepen, Kevin
Marchal, Kathleen #
Issue Date: 2014
Publisher: BioMed Central
Series Title: BMC Genomics vol:15 issue:1 pages:207
Article number: 10.1186/1471-2164-15-207
Abstract: Bulk segregant analysis (BSA) coupled to high throughput sequencing is a powerful method to map genomic regions related with phenotypes of interest. It relies on crossing two parents, one inferior and one superior for a trait of interest. Segregants displaying the trait of the superior parent are pooled, the DNA extracted and sequenced. Genomic regions linked to the trait of interest are identified by searching the pool for overrepresented alleles that normally originate from the superior parent. BSA data analysis is non-trivial due to sequencing, alignment and screening errors.
URI: 
ISSN: 1471-2164
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre of Microbial and Plant Genetics
Molecular Microbiology and Biotechnology Section - miscellaneous
× corresponding author
# (joint) last author

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