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Title: Deciphering the role of the thermodynamic and kinetic stabilities of SH3 domains on their aggregation inside bacteria
Authors: Castillo Cano, Virginia *
Espargaró, Alba *
Gordo, Veronica
Vendrell, Josep
Ventura, Salvador # ×
Issue Date: Dec-2010
Publisher: WILEY-VCH Verlag
Series Title: Proteomics vol:10 issue:23 pages:4172-85
Article number: 10.1002/pmic.201000260
Abstract: The formation of insoluble deposits by globular proteins underlies the onset of many human diseases. Recent studies suggest a relationship between the thermodynamic stability of proteins and their in vivo aggregation. However, it has been argued that, in the cell, the occurrence of irreversible aggregation might shift the system from equilibrium, in such a way that it could be the rate of unfolding and associated kinetic stability instead of the conformational stability that controls protein deposition. This is an important but difficult to decipher question, because kinetic and thermodynamic stabilities appear usually correlated. Here we address this issue by comparing the in vitro folding kinetics and stability features of a set of non-natural SH3 domains with their aggregation properties when expressed in bacteria. In addition, we compare the in vitro stability of the isolated domains with their effective stability in conditions that mimic the cytosolic environment. Overall, the data argue in favor of a thermodynamic rather than a kinetic control of the intracellular aggregation propensities of small globular proteins in which folding and unfolding velocities largely exceed aggregation rates. These results have implications regarding the evolution of proteins.
URI: 
ISSN: 1615-9853
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
* (joint) first author
× corresponding author
# (joint) last author

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