Title: Hydrogenase in Bradyrhizobium japonicum: Occurrence, Genetics and Effect on Plant Growth
Authors: Van Soom, Caroline ×
Ramjanek, N
Neves, C
Vanderleyden, Jozef #
Issue Date: 1993
Publisher: Rapid Science Publishers
Series Title: World Journal of Microbiology and Biotechnology vol:9 pages:615-624
Abstract: A region of 15 kbp DNA located on the chromosome of Bradyrhizobium japonicum is essential for a Hup+ phenotype and contains the hydrogenase structural genes. During the last few years, other genes with various functions related to hydrogenase oxidation, such as Ni2+ incorporation into the hydrogenase enzyme, electron transport from hydrogenase to O2, and regulation of hydrogenase expression in free-living conditions, have been identified in this region. A region in front of the hydrogenase structural genes is necessary for transcriptional regulation of hydrogenase expression by O2, H-2 and Ni2+. In addition, the N2 fixation system and the tertiary structure of the chromosome seem to be involved in the expression of the hydrogenase genes. The effect of legume inoculation with Hup+ rhizobia has been evaluated. The possible benefits of a Hup+ phenotype, such as the regeneration of chemical energy in the form of ATP or reductants, and the removal of O2 and H-2 from the active site of the nitrogenase enzyme where they might inhibit the nitrogenase reaction, are discussed. The data indicate that a H-2-uptake system is beneficial in soybean nodules, but host plant and environmental factors may interfere with the effects of H-2 cycling in the plant.
ISSN: 0959-3993
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre of Microbial and Plant Genetics
× corresponding author
# (joint) last author

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