Title: Effect of oxygen on the nitrogen fixation activity and expression of the Azospirillum brasilense nifH gene in various plant-associated diazotrophs
Authors: Vande Broek, Ann ×
Keijers, V.
Vanderleyden, Jozef #
Issue Date: 1996
Publisher: Int Science Services/Balaban Publishers
Series Title: Symbiosis vol:21 pages:25-40
Abstract: As demonstrated previously (Vande Broek et al., 1993), the ambient oxygen tension can severely reduce associative N-2 fixation by plant-associated diazotrophs such as Azospiuillum buasilense. To identify associative N-2 fixers that possess a more oxygen-tolerant nitrogen-fixation system, we analyzed the expression of an Azospiuillum buasilense nifH-gusA fusion and the acetylene reduction activity as a function of the oxygen concentration in eight aerobic associative diazotrophs, namely Acetobacter diazotrophicus, Alcaligenes faecalis, Azoarcus indigens, Azorhizophilus paspali, Azospirillum brasilense, Azospirillum irakense, Burkholderia vietnamiensis and Herbaspirillum seropedicae. On the basis of the maximum oxygen concentration at which activation of the nifH fusion and acetylene reduction were still observed, these organisms were classified into three groups. In order of increasing oxygen tolerance, these groups are: (i) Acetobacter diazotrophicus, Alcaligenes faecalis, Azospirillum buasilense, Azospirillum iuakense, Burkholderia vietnamiensis and Herbaspirillum seropedicae (maximum oxygen tension for acetylene reduction between 2.0 and 3.0%); (ii) Azoarcus indigens (maximum oxygen tension for acetylene reduction 6.5%); and (iii) Azorhizophilus paspali (maximum oxygen:tension for acetylene reduction >8.5%).
ISSN: 0334-5114
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre of Microbial and Plant Genetics
× corresponding author
# (joint) last author

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