Title: A GFP promoter fusion library for the study of Salmonella biofilm formation and the mode of action of biofilm inhibitors
Authors: Robijns, Stijn
Roberfroid, Stefanie
Van Puyvelde, Sandra
De Pauw, Bruno
Uceda Santamaría, E.
De Weerdt, Ami
De Coster, David
Hermans, Kim
De Keersmaecker, Sigrid
Vanderleyden, Jos
Steenackers, Hans # ×
Issue Date: 2014
Publisher: Harwood Academic Publishers
Series Title: Biofouling vol:30 issue:5 pages:605-25
Abstract: Salmonella, an important foodborne pathogen, forms biofilms in many different environments. The composition of these biofilms differs depending on the growth conditions, and their development is highly coordinated in time. To develop efficient treatments, it is therefore essential that biofilm formation and its inhibition be understood in different environments and in a time-dependent manner. Many currently used techniques, such as transcriptomics or proteomics, are still expensive and thus limited in their application. Therefore, a GFP-promoter fusion library with 79 important Salmonella biofilm genes was developed (covering among other things matrix production, fimbriae and flagella synthesis, and c-di-GMP regulation). This library is a fast, inexpensive, and easy-to-use tool, and can therefore be conducted in different experimental setups in a time-dependent manner. In this paper, four possible applications are highlighted to illustrate and validate the use of this reporter fusion library.
ISSN: 0892-7014
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre of Microbial and Plant Genetics
Department of Business and Management Studies - UC Leuven
× corresponding author
# (joint) last author

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