Title: Exploring nucleo-cytoplasmic large DNA viruses in Tara Oceans microbial metagenomes
Authors: Hingamp, Pascal ×
Grimsley, Nigel
Acinas, Silvia G
Clerissi, Camille
Subirana, Lucie
Poulain, Julie
Ferrera, Isabel
Sarmento, Hugo
Villar, Emilie
Lima Mendez, Gipsi
Faust, Karoline
Sunagawa, Shinichi
Claverie, Jean-Michel
Moreau, Hervé
Desdevises, Yves
Bork, Peer
Raes, Jeroen
de Vargas, Colomban
Karsenti, Eric
Kandels-Lewis, Stefanie
Jaillon, Olivier
Not, Fabrice
Pesant, Stéphane
Wincker, Patrick
Ogata, Hiroyuki #
Issue Date: Sep-2013
Publisher: Nature Publishing Group
Series Title: The ISME Journal vol:7 issue:9 pages:1678-1695
Article number: 10.1038/ismej.2013.59
Abstract: Nucleo-cytoplasmic large DNA viruses (NCLDVs) constitute a group of eukaryotic viruses that can have crucial ecological roles in the sea by accelerating the turnover of their unicellular hosts or by causing diseases in animals. To better characterize the diversity, abundance and biogeography of marine NCLDVs, we analyzed 17 metagenomes derived from microbial samples (0.2-1.6 μm size range) collected during the Tara Oceans Expedition. The sample set includes ecosystems under-represented in previous studies, such as the Arabian Sea oxygen minimum zone (OMZ) and Indian Ocean lagoons. By combining computationally derived relative abundance and direct prokaryote cell counts, the abundance of NCLDVs was found to be in the order of 10(4)-10(5) genomes ml(-1) for the samples from the photic zone and 10(2)-10(3) genomes ml(-1) for the OMZ. The Megaviridae and Phycodnaviridae dominated the NCLDV populations in the metagenomes, although most of the reads classified in these families showed large divergence from known viral genomes. Our taxon co-occurrence analysis revealed a potential association between viruses of the Megaviridae family and eukaryotes related to oomycetes. In support of this predicted association, we identified six cases of lateral gene transfer between Megaviridae and oomycetes. Our results suggest that marine NCLDVs probably outnumber eukaryotic organisms in the photic layer (per given water mass) and that metagenomic sequence analyses promise to shed new light on the biodiversity of marine viruses and their interactions with potential hosts.
ISSN: 1751-7362
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

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