Title: Development and application of a dapB-based in vivo expression technology system to study colonization of rice by the endophytic nitrogen-fixing bacterium Pseudomonas stutzeri A15
Authors: Rediers, Hans ×
Bonnecarrère, V.
Rainey, P. B.
Hamonts, K.
Vanderleyden, Jozef
De Mot, René #
Issue Date: 2003
Publisher: American Society for Microbiology (ASM)
Series Title: Applied and Environmental Microbiology vol:69 pages:6864-6874
Abstract: Pseudomonas stutzeri A15 is a nitrogen-fixing bacterium isolated from paddy rice. Strain A15 is able to colonize and infect rice roots. This strain may provide rice plants with fixed nitrogen and hence promote plant growth. In this article, we describe the use of dapB-based in vivo expression technology to identify P. stutzeri A15 genes that are specifically induced during colonization and infection (cii). We focused on the identification of P. stutzeri A15 genes that are switched on during rice root colonization and are switched off during free-living growth on synthetic medium. Several transcriptional fusions induced in the rice rhizosphere were isolated. Some of the corresponding genes are involved in the stress response, chemotaxis, metabolism, and global regulation, while others encode putative proteins with unknown functions or without significant homology to known proteins.
ISSN: 0099-2240
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre of Microbial and Plant Genetics
Microbial and Molecular Systems - miscellaneous
Bioengineering Technology TC, Technology Campus De Nayer Sint-Katelijne-Waver
Technologiecluster Bioengineering Technologie
× corresponding author
# (joint) last author

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