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Title: Structural and functional analysis of the Rhizobium leguminosarum biovar phaseoli CNPAF512 fixLJ genes
Authors: D'Hooghe, Inge ×
Michiels, Jan
Vlassak, Katrien
Verreth, Christel
Waelkens, Franciska
Vanderleyden, Jozef #
Issue Date: 1995
Publisher: Springer-Verlag Heidelberg
Series Title: Molecular & General Genetics vol:249 pages:117-126
Abstract: The fixLJ genes of Rhizobium leguminosarum biovar phaseoli CNPAF512 were identified by DNA hybridization of a genomic library with an internal fragment of the Rhizobium meliloti fixJ gene. The nucleotide sequence was determined and the corresponding amino acid sequence was aligned with the amino acid sequences of the FixL proteins of R. meliloti, Bradyrhizobium japonicum and Azorhizobium caulinodans. While the FixJ protein and the carboxy-terminal part of the FixL protein are highly homologous to the other FixL and FixJ proteins, the homology in the central heme-binding, oxygen-sensing domain and in the amino-terminal domain of FixL is very low. The R. leguminosarum bv. phaseoli FixL protein does not contain the heme-binding motif defined for the previously described FixL proteins. R. leguminosarum bv. phaseoli fixLJ and fixJ mutants were constructed. These mutants can still fix nitrogen, albeit at a reduced level. Expression analysis of nifA-gusA and nifH-gusA fusions in the constructed mutants revealed that the R. leguminosarum bv. phaseoli fixLJ genes are involved in microaerobic nifH expression but not in nifA expression.
ISSN: 0026-8925
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre of Microbial and Plant Genetics
Microbial and Molecular Systems - miscellaneous
Bioengineering Technology TC, Technology Campus De Nayer Sint-Katelijne-Waver
Technologiecluster Bioengineering Technologie
× corresponding author
# (joint) last author

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