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Title: PKA and Sch9 control a molecular switch important for the proper adaptation to nutrient availability
Authors: Roosen, Johnny
Engelen, Kristof
Marchal, Kathleen
Mathys, Janick
Griffioen, Johan
Cameroni, E
Thevelein, Johan
De Virgilio, C
De Moor, Bart
Winderickx, Joris # ×
Issue Date: Feb-2005
Publisher: Blackwell Science
Series Title: Molecular Microbiology vol:55 issue:3 pages:862-880
Abstract: AB - In the yeast Saccharomyces cerevisiae, PKA and Sch9 exert similar physiological roles in response to nutrient availability. However, their functional redundancy complicates to distinguish properly the target genes for both kinases. In this article, we analysed different phenotypic read-outs. The data unequivocally showed that both kinases act through separate signalling cascades. In addition, genome-wide expression analysis under conditions and with strains in which either PKA and/or Sch9 signalling was specifically affected, demonstrated that both kinases synergistically or oppositely regulate given gene targets. Unlike PKA, which negatively regulates stress-responsive element (STRE)- and post-diauxic shift (PDS)-driven gene expression, Sch9 appears to exert additional positive control on the Rim15-effector Gis1 to regulate PDS-driven gene expression. The data presented are consistent with a cyclic AMP (cAMP)-gating phenomenon recognized in higher eukaryotes consisting of a main gatekeeper, the protein kinase PKA, switching on or off the activities and signals transmitted through primary pathways such as, in case of yeast, the Sch9-controlled signalling route. This mechanism allows fine-tuning various nutritional responses in yeast cells, allowing them to adapt metabolism and growth appropriately
Description: \emph{Molecular Microbiology}, vol. 55, no. 3, Feb. 2005
ISSN: 0950-382X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Physiology of Plants and Micro-organisms Section - miscellaneous
Centre of Microbial and Plant Genetics
ESAT - STADIUS, Stadius Centre for Dynamical Systems, Signal Processing and Data Analytics
Laboratory for Functional Biology (-)
Molecular Microbiology and Biotechnology Section - miscellaneous
× corresponding author
# (joint) last author

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